Review



iscripttm gdna clear cdna synthesis kit  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Bio-Rad iscripttm gdna clear cdna synthesis kit
    Iscripttm Gdna Clear Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscripttm+gdna+clear+cdna+synthesis+kit/iScript+cDNA+Synthesis+Kit/pmc13101709-246-10-16
    Average 99 stars, based on 48025 article reviews
    iscripttm gdna clear cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    cDNA Synthesis:

    Article Title: Puckering astringent compounds target Gαq-coupled receptors and induce store-operated calcium entry via Orai channels in the human tongue cell line HSC-3
    Article Snippet: Then the RNA from the cells was isolated using the Monarch® Total RNA Miniprep Kit (New England Biolabs GmbH, Germany) according to the manufacturer's instructions. .. The iSCriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories GmbH, Germany) was used for DNase digestion and cDNA synthesis according to the manufacturer's instructions in a C1000 Touch Thermal Cycler (Bio-Rad Laboratories GmbH, Germany). .. RT-qPCR was carried out with 50 ng of cDNA and the Advanced Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, Germany) in the C1000 Touch Thermal Cycler with a CFX96TM Real-Time System (Bio-Rad Laboratories GmbH, Germany).

    Article Title: CuiT is a Cu importer required for metal homeostasis in Salmonella enterica
    Article Snippet: Aliquots of 0.5 mL were stabilized with RNA protect Bacteria Reagent (Qiagen), RNA was isolated with RNeasy Mini Kit (Qiagen), treated with DNase (Qiagen). .. RNA (500 ng) was used for cDNA synthesis using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad). .. Quantitative PCR (qPCR) were carried out using 25 ng of cDNA in a LightCycler® 96 System (Roche) with AzuraViewTM GreenFast qPCR Blue Mix LR (Azura Genomics) in a 20 μl final volume, using 0.8 μM of each primer. rnpB was used as the reference gene.

    Article Title: Machine learning-based transcriptomic analysis of differentially expressed genes in the epithelium and stroma of keratoconus.
    Article Snippet: .. Complementary DNA (cDNA) was synthesized using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA). .. The primer sequences are listed in Supplementary Table 3. qRT–PCR was conducted using fivefold diluted cDNA and the SYBR Green Pro Taq HS qPCR Kit ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS (AG11701; Accurate Biotechnology, China) in a 20 μL reaction volume containing 10 μL of 2× SYBR Green Pro Taq HS Premix, 0.4 μL each of forwards and reverse primers (10 μM), and RNase-free water.

    Article Title: Stage-specific CHI3L1/YKL-40 signaling controls generation of oligodendrocyte precursor cells through IL13Rα2-mediated ferroptosis
    Article Snippet: Acetylcysteine (N-Acetylcysteine, MCE: HY-B0215), Azeliragon (TTP488, MCE: HY-50682), Deferoxamine Mesylate (MCE: HY-B0988), Aspartic acid (MCE: HY-42068), FPS-ZM1 (MCE: HY-19370), 1,5-Isoquinolinediol (MCE: HY-W015422), Z-VAD-FMK (MCE: HY-16658B), Necrostatin-1 (MCE: HY-15760), Ferrostatin-1 (MCE: HY-100579), Disulfiram (Tetraethylthiuram disulfide, MCE: HY-B0240) were incubated in culture as indicated. .. mRNA was harvested with the Qiagen RNeasy Mini Kit (Qiagen #74104). cDNA was synthesized following the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035). .. SYBR green probes (obtained from Bio-Rad) and FAM probes (obtained from iDT) targeting genes of interest were applied to evaluate gene expression under the CFX96 Touch Real-Time PCR Detection System.

    Article Title: VPS13 has an important role in female germline development in Arabidopsis.
    Article Snippet: Extraction, cDNA library preparation, and sequencing for total RNA-seq and short RNA-seq Total RNA was extracted using mirVanaTM miRNA Isolation Kit, with phenol by Thermofisher. .. RNA samples were treated for gDNA contamination using the Ambion DNA-freeTM DNA Removal Kit and retrotranscribed with iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories). ..

    Article Title: Genetic Modulation of Wound Healing Pathways and Postoperative Risk in Plastic and Reconstructive Surgery: A Cohort Study.
    Article Snippet: Transcriptional activity of IL6, CCL2, MAPK1, MAPK8, IL10, MMP9, COL1A1 and COL3A1 genes in the peripheral blood leukocytes was assessed using a PCR with iTaq Universal SYBR Green Supermix (SMX500; Thermo Fisher Scientific Inc., Bio-Rad Laboratories, Waltham, MA, USA). .. Total RNA was isolated using the GeneAll® Hybrid-RTM Blood RNA Kit (GeneAll Biotechnology, Seoul, Republic of Korea) according to the manufacturer’s instructions and reverse-transcribed into cDNA using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA). ..

    Article Title: Genetic Modulation of Wound Healing Pathways and Postoperative Risk in Plastic and Reconstructive Surgery: A Cohort Study
    Article Snippet: Transcriptional activity of IL6, CCL2, MAPK1, MAPK8, IL10, MMP9, COL1A1 and COL3A1 genes in the peripheral blood leukocytes was assessed using a PCR with iTaq Universal SYBR Green Supermix (SMX500; Thermo Fisher Scientific Inc., Bio-Rad Laboratories, Waltham, MA, USA). .. Total RNA was isolated using the GeneAll ® Hybrid-RTM Blood RNA Kit (GeneAll Biotechnology, Seoul, Republic of Korea) according to the manufacturer’s instructions and reverse-transcribed into cDNA using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA). ..

    Article Title: Flavonoid and isoflavonoid biosynthetic gene expressions is affected by MYB36 transcription factor deficiency in Lotus japonicus.
    Article Snippet: .. For RT-PCR, cDNA was synthesized from 1 μg of total RNA using the iScriptTM gDNA clear cDNA synthesis Kit (Bio-Rad), which includes DNAse treatment for removal of genomic DNA contamination. ..

    Synthesized:

    Article Title: Machine learning-based transcriptomic analysis of differentially expressed genes in the epithelium and stroma of keratoconus.
    Article Snippet: .. Complementary DNA (cDNA) was synthesized using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA). .. The primer sequences are listed in Supplementary Table 3. qRT–PCR was conducted using fivefold diluted cDNA and the SYBR Green Pro Taq HS qPCR Kit ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS (AG11701; Accurate Biotechnology, China) in a 20 μL reaction volume containing 10 μL of 2× SYBR Green Pro Taq HS Premix, 0.4 μL each of forwards and reverse primers (10 μM), and RNase-free water.

    Article Title: Stage-specific CHI3L1/YKL-40 signaling controls generation of oligodendrocyte precursor cells through IL13Rα2-mediated ferroptosis
    Article Snippet: Acetylcysteine (N-Acetylcysteine, MCE: HY-B0215), Azeliragon (TTP488, MCE: HY-50682), Deferoxamine Mesylate (MCE: HY-B0988), Aspartic acid (MCE: HY-42068), FPS-ZM1 (MCE: HY-19370), 1,5-Isoquinolinediol (MCE: HY-W015422), Z-VAD-FMK (MCE: HY-16658B), Necrostatin-1 (MCE: HY-15760), Ferrostatin-1 (MCE: HY-100579), Disulfiram (Tetraethylthiuram disulfide, MCE: HY-B0240) were incubated in culture as indicated. .. mRNA was harvested with the Qiagen RNeasy Mini Kit (Qiagen #74104). cDNA was synthesized following the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035). .. SYBR green probes (obtained from Bio-Rad) and FAM probes (obtained from iDT) targeting genes of interest were applied to evaluate gene expression under the CFX96 Touch Real-Time PCR Detection System.

    Article Title: Flavonoid and isoflavonoid biosynthetic gene expressions is affected by MYB36 transcription factor deficiency in Lotus japonicus.
    Article Snippet: .. For RT-PCR, cDNA was synthesized from 1 μg of total RNA using the iScriptTM gDNA clear cDNA synthesis Kit (Bio-Rad), which includes DNAse treatment for removal of genomic DNA contamination. ..

    Isolation:

    Article Title: Genetic Modulation of Wound Healing Pathways and Postoperative Risk in Plastic and Reconstructive Surgery: A Cohort Study.
    Article Snippet: Transcriptional activity of IL6, CCL2, MAPK1, MAPK8, IL10, MMP9, COL1A1 and COL3A1 genes in the peripheral blood leukocytes was assessed using a PCR with iTaq Universal SYBR Green Supermix (SMX500; Thermo Fisher Scientific Inc., Bio-Rad Laboratories, Waltham, MA, USA). .. Total RNA was isolated using the GeneAll® Hybrid-RTM Blood RNA Kit (GeneAll Biotechnology, Seoul, Republic of Korea) according to the manufacturer’s instructions and reverse-transcribed into cDNA using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA). ..

    Article Title: Genetic Modulation of Wound Healing Pathways and Postoperative Risk in Plastic and Reconstructive Surgery: A Cohort Study
    Article Snippet: Transcriptional activity of IL6, CCL2, MAPK1, MAPK8, IL10, MMP9, COL1A1 and COL3A1 genes in the peripheral blood leukocytes was assessed using a PCR with iTaq Universal SYBR Green Supermix (SMX500; Thermo Fisher Scientific Inc., Bio-Rad Laboratories, Waltham, MA, USA). .. Total RNA was isolated using the GeneAll ® Hybrid-RTM Blood RNA Kit (GeneAll Biotechnology, Seoul, Republic of Korea) according to the manufacturer’s instructions and reverse-transcribed into cDNA using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA). ..

    Reverse Transcription:

    Article Title: Genetic Modulation of Wound Healing Pathways and Postoperative Risk in Plastic and Reconstructive Surgery: A Cohort Study.
    Article Snippet: Transcriptional activity of IL6, CCL2, MAPK1, MAPK8, IL10, MMP9, COL1A1 and COL3A1 genes in the peripheral blood leukocytes was assessed using a PCR with iTaq Universal SYBR Green Supermix (SMX500; Thermo Fisher Scientific Inc., Bio-Rad Laboratories, Waltham, MA, USA). .. Total RNA was isolated using the GeneAll® Hybrid-RTM Blood RNA Kit (GeneAll Biotechnology, Seoul, Republic of Korea) according to the manufacturer’s instructions and reverse-transcribed into cDNA using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA). ..

    Article Title: Genetic Modulation of Wound Healing Pathways and Postoperative Risk in Plastic and Reconstructive Surgery: A Cohort Study
    Article Snippet: Transcriptional activity of IL6, CCL2, MAPK1, MAPK8, IL10, MMP9, COL1A1 and COL3A1 genes in the peripheral blood leukocytes was assessed using a PCR with iTaq Universal SYBR Green Supermix (SMX500; Thermo Fisher Scientific Inc., Bio-Rad Laboratories, Waltham, MA, USA). .. Total RNA was isolated using the GeneAll ® Hybrid-RTM Blood RNA Kit (GeneAll Biotechnology, Seoul, Republic of Korea) according to the manufacturer’s instructions and reverse-transcribed into cDNA using the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA). ..



    Similar Products

    iscripttm gdna clear cdna synthesis kit  (Bio-Rad)
    99
    Bio-Rad iscripttm gdna clear cdna synthesis kit
    Iscripttm Gdna Clear Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscripttm+gdna+clear+cdna+synthesis+kit/iScript+cDNA+Synthesis+Kit/pmc13101709-246-10-16
    Average 99 stars, based on 1 article reviews
    iscripttm gdna clear cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results